Laser Diode Thermal Desorption Application Notes

Pharmaceutical

 

The pharmaceutical drug discovery and development lifecycle averages in excess of 10 years, and over $1 billion dollars to bring a drug to market. Assessment of physiochemical and pharmacological properties is now conducted at very early stages of drug discovery for the purpose of accelerating the conversion of hits and leads into qualified development candidates.

 

Laser Diode Thermal Desorption (LDTD) is a “next generation” technology which features liquid samples deposited directly onto the LazWell plate, with throughput speeds routinely under 9 seconds per sample, and even faster using acoustic dispersion technology. Application notes and posters, articles and presentations concentrate on CYP Inhibition, HT-ADME and PK as well as High-Throughput Screening (HTS) support.

 

Key Parameters for a Good Liquid Transfer to Lazwell Plates

KEYWORDS:

  • Lazwell
  • LDTD
  • Liquid Transfer


Phosphatidylcholine Profile Evaluation in Human Plasma at 8 Seconds per Sample using the LDTD-MS/MS Technique

KEYWORDS: 

  • LDTD-MS/MS


Continuing the Quest for a Mass Spectrometry-Based Plate Reader: Evaluating Laser Diode Thermal Desorption (LDTD) Coupled with Nanoliter Dispensing for HT-ADME and Other HTS Applications

  • Partnership: Bristol-Myers Squibb
  • Molecules: 
  • Matrix:
  • Extraction Method: 
  • Instrumentation: LDTD/MS-MS
  • No. Code: Presentation

    Dipyridamole High-Throughput Analysis of Drug Metabolism Cell-Based Assays Using LDTD-MS/MS

     

    • Partnership: N/A
    • Molecules: Dipyridamole
    • Matrix:
    • Extraction Method: Protein precipitation
    • Instrumentation: SCIEX 5500 QTrap and LDTD: LUXON
    • No. Code:

     

    Label Free High-Throughput Screening of Amino Acid Based Assays

    • Partnership: Phytronix Technologies
    • Molecules:
    • Matrix:
    • Extraction Method:
    • Instrumentation: SCIEX 5500 QTRAP and LUXON
    • No. Code:

      Label-Free High Throughput Screening of Steroid using LDTD-MS/MS

      • Partnership: n/a
      • Molecules: 7-25-Dihydroxycholesterol
      • Matrix: Cell culture buffer
      • Extraction Method: Automated Liquid-Liquid extraction
      • Instrumentation: SCIEX 5500 Qtrap
      • No. Code: n/a

      Uninterrupted Label-Free High-Throughput Screening of CYP Using Acoustic Deposition Coupled with LDTD-MS/MS

       

      • Partnership: N/A
      • Molecules: CYP Probe substrates
      • Matrix: Enzyme buffer
      • Extraction Method: Sample Dilution
      • Instrumentation: SCIEX 5500 Qtrap
      • No. Code: N/A

       

      Enabling Nanoliter Sample Size in Mass Spectrometry Analysis Using Laser Diode Thermal Desorption: Application to Pharmaceutical Matrices

       

      • Partnership: N/A
      • Molecules: Dextrorphan OH-Testosterone and OH-Midazolam, and Plasma-spiked with Clozapine
      • Matrix: Human plasma
      • Extraction Method: Plasma protein; Precipitation and CYP culture media organic quench
      • Instrumentation: AB Sciex 5500 QTrap
      • No. Code: 153

       

      High-Throughput Analysis of Anti-fungal Paraben in Personal Care Products at 9 Seconds per Sample Using LDTD-MS/MS

      • Partnership: Universite Laval (Quebec, Canada)
      • Molecules: Paraben: Methyl, Ethyl, Propyl, Butyl
      • Matrix:
      • Extraction Method: Sample dilution in HCI, LLE
      • Instrumentation: AB Sciex 5500 QTRAP
      • No. Code: 237

        LDTD and High Resolution Mass Spectrometry Workflows to Enhance Sample Throughput for the Automated In-Vitro ADME Laboratory

        • Partnership: AMGEN
        • Molecules:
        • Matrix:
        • Extraction Method:
        • Instrumentation:
        • No. Code:

          Strategy for Predicting Molecular Coverage and Enhancing Successful Analysis for CACO2 Studies in High-Throughput LDTD-MS/MS

          • Partnership: N/A
          • Molecules: Acidic Groups
          • Matrix: Buffer
          • Extraction Method: N/A
          • Instrumentation: UPLC
          • No. Code: WP626

            Acyl Glucuronides Separation Method for Plasma Analysis by LDTD MS/MS in Less Than 9 Seconds per Sample

            • Partnership: N/A
            • Molecules: Acyl Glucuronides
            • Matrix: Plasma
            • Extraction Method: SALLE, LLE, Protein Precipitation
            • Instrumentation: Thermo Vantage MS/MS
            • No. Code: WP634

            Generic SLE Extraction Method for Ultra-fast Drug Screening in Saliva Using LDTD-MS/MS Analysis

             

            • Partnership: N/A
            • Molecules:
            • Matrix: Oral fluids
            • Extraction Method: Supported Liquid Extraction (SLE)
            • Instrumentation: AB Sciex Qtrap 5500
            • No. Code: N/A

             

            LDTD-MS/MS Method Validation According to FDA Regulation

            • Partnership: None
            • Molecules: Dextromethorphan
            • Matrix: Human Plasma
            • Extraction Method: Protein Precipitation
            • Instrumentation: Waters Xevo TQMS
            • No. Code: 1105

              LDTD-MS/MS for CYP 1A2 / 2C9 / 2D6 / 3A4 Inhibition Assays in 18 Minutes

              • Partnership: None
              • Molecules: miconazole, ketoconazole, nicardepine, nifedipine, mifepristone, clarithromicyn, a-naphtoflavone, quinidine, clozapine, imipramine, desipramine, bufurolol, sulfaphenazole, fluvoxamine, genistein
              • Matrix: CYP 1A2/2C9/2D6/3A4 inhibition assays
              • Extraction Method: Liquid-Liquid
              • Instrumentation: API 4000 triple quadrupole
              • No. Code: 713

                4.8 Seconds Sample to Sample Analysis in ADME Using LDTD TripleTOF™ 5600 System

                • Partnership: AB Sciex
                • Molecules: OH-Midazolam, 4OH-Tolbutamide, 1OH-Bufurolol, 4OH-Mephenytoin, OH-Buproprion, Resorufin, 7OH-Coumarin
                • Matrix: Caco-2 Buffer Solution
                • Extraction Method: Protein Precipitation + Salting Out Dilution
                • Instrumentation: AbSciex TripleTOF 5600
                • No. Code: PHAB_1107

                  High-Throughput LDTD-MS/MS IC50 Determination of CYP Inhibition in HLM

                  • Partnership: None
                  • Molecules: Acetaminophen, 4-OH-Diclofenac, Dextrorphan, 1-OH-Midazolam
                  • Matrix: Human Liver Microsomes
                  • Extraction Method: Liquid-Liquid
                  • Instrumentation: AB Sciex Qtrap 5500
                  • No. Code: 1217

                    Salt-Assisted Liquid-Liquid Extraction (SALLE) Technique for CYP Inhibition Using LDTD-TripleTOF 5600 System

                    • Partnership: None
                    • Molecules: 4OH-Diclofenac, Dextrorphan, 1OH-Midazolam
                    • Matrix: Human Liver Microsomes
                    • Extraction Method: Protein Precipitaion + Salt Induced phase separation
                    • Instrumentation: AbSciex TripleTOF 5600
                    • No. Code: 1201

                      CACO-2 Permeability Coefficient Determination in LDTD-MS/MS

                      • Partnership: Sanofi-Aventis
                      • Molecules: Dextromethorphan, Propanolol, Imipramine, Diltiazem, Alfusozin, Hydroxyzine, Desloratadine, Atenolol, Nadolol, Sulpiride, Encatropine
                      • Matrix: Bovine Serum Albumine
                      • Extraction Method: Incubation + Protein Precipitation
                      • Instrumentation: Thermo TSQ Vantage Triple Quadrupole
                      • No. Code: 1003

                        High-Throughput Workflow Analysis: Dextrorphan in Human Plasma

                        • Partnership: None
                        • Molecules: Dextrorphan
                        • Matrix: Human Plasma
                        • Extraction Method: Liquid-Liquid
                        • Instrumentation: Agilent 6410
                        • No. Code: 1002

                          High-Throughput LDTD-MS/MS IC50 Determination of CYP Inhibition in HLM

                          • Partnership: None
                          • Molecules: Phenacetin, Diclofenac, Tolbutamide, S-Mephenytoin, Bufurolol, Dextromethorphan, Midazolam, Testosterone, Nifepidine
                          • Matrix: Human Liver Microsomes
                          • Extraction Method: Protein Precipitation
                          • Instrumentation: Thermo TSQ Vantage Triple Quadrupole
                          • No. Code: 0714

                            LDTD-MS/MS Analysis in 9 Seconds: Quantification of Mifepristone in Mouse Plasma

                            • Partnership: None
                            • Molecules: Mifepristone, MifA-1, MifA-2
                            • Matrix: Mouse Plasma
                            • Extraction Method: Liquid-Liquid
                            • Instrumentation: Thermo TSQ Quantum Ultra AM
                            • No. Code: 0710

                              Midazolam and 1-Hydroxymidazolam in Human Plasma : LDTD-MS/MS Analysis in 8 Seconds

                              • Partnership: None
                              • Molecules: Midazolam, 1OH-Midazolam
                              • Matrix: Plasma
                              • Extraction Method: Liquid-Liquid
                              • Instrumentation: Thermo TSQ Quantum Ultra AM
                              • No. Code: 0709

                                LDTD-MS/MS in 1.8 Seconds with RSD of 2.4%: Paracetamol in Human Plasma Crash

                                • Partnership: None
                                • Molecules: Paracetamol, Acetaminophen
                                • Matrix: Stripped Human Plasma
                                • Extraction Method: Protein Precipitation
                                • Instrumentation: Thermo TSQ Quantum Ultra AM
                                • No. Code: 0708

                                  High-Throughput Analysis of 960 Reserpine Samples in 1.9 Hours

                                  • Partnership: None
                                  • Molecules: Reserpine
                                  • Matrix: Human Plasma
                                  • Extraction Method: Protein Precipitation
                                  • Instrumentation: Thermo TSQ Quantum Ultra AM
                                  • No. Code: 0706

                                    LDTD-MS/MS Method Validation for Quantitative Analysis of Small Molecule in DBS

                                    • Partnership: None
                                    • Molecules: Dextrorphan
                                    • Matrix: Human Blood
                                    • Extraction Method: Dry Blood Spot
                                    • Instrumentation: Thermo TSQ Vantage Triple Quadrupole
                                    • No. Code: 1102

                                      Dry Blood spot of Dextrorphan on FTA-DMPK-A and FTA-DMPK-C cards: LDTD-MS/MS Analysis in 10 seconds

                                      • Partnership: None
                                      • Molecules: Dextrorphan
                                      • Matrix: Human Blood
                                      • Extraction Method: Dry Blood Spot
                                      • Instrumentation: Thermo TSQ Vantage Triple Quadrupole
                                      • No. Code: 1101